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KMID : 0368419920350010091
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Journal of Plant Biology
1992 Volume.35 No. 1 p.91 ~ p.95
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Changes of Sucrose Content and Invertase Activity in Leaves of Barley Seedlings under Low Temperature
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李明愛/Lee, Myung Ae
孟柱善/金相九/權寧命/Maeng, Ju Sun/Kim, Sang Gu/Kwon, Young Myung
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Abstract
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Changes of contents of reducing sugar and sucrose and activities of sucrose-phosphate synthase, sucrose synthease and invertase from the leaves of barley (Hordeum vulgare L. cv. Chalssal) seedlings grown at 4℃ were investigated, and the property of acid invertase were also examined. In the seedlings grown at 4℃ for 3 days, the contents of reducing sugar and sucrose were increased to 1.3 and 2.4 times, respectively. Activity of acid invertase was decreased markedly by cold treatment while the activities of sucrosephosphate synthase, sucrose synthase, and alkaline invertase were not changed. In acid phosphatase purified partially by ammonium sulfate fractionation and DEAE-Sephacel column chromatography, the K_m value for sucrose was 9.5 mM and the optimum pH and temperature was 5.5 and 35℃ respectively. This enzyme was supposed to be β-fructosidase by studies on the substrate specificity and the molecular weight was estimated to be 63 Kd by Sephadex G-200 gel chromatography.
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